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dr yi zhang  (Addgene inc)


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  • 91

    Structured Review

    Addgene inc dr yi zhang
    Dr Yi Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dr+yi+zhang/pcDNA-Flag-Tet3+(Plasmid+%2360940)/pmc12310658-89-11-15
    Average 91 stars, based on 5 article reviews
    dr yi zhang - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Histone methyltransferase DOT1L coordinates AR and MYC stability in prostate cancer.
    Article Snippet: .. MSCB-hDot1Lwt was a gift from Dr. Yi Zhang (Addgene plasmid # 74173). pcDNA3.1 plasmid (EV) and Flag tagged AR plasmid was kindly provided by Dr. Meejeon Roh. pSMP-Luc and pSMP-Dot1L_1 was a gift from George Daley (Addgene plasmid # 36339 and plasmid # 36394). ..

    Article Title: Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia
    Article Snippet: .. For Tet3 overexpression, we used a previously reported Tet3 plasmid by Dr. Yi Zhang, pcDNA-Flag-Tet3 (Addgene plasmid #60940; RRID:Addgene_60940 ) ( ); a pBluescript II KS(+) plasmid was used as a control plasmid for the transfection procedure. .. Lipofectamine 3,000 reagent (Thermo Fisher Scientific) was used for transfection, with 5 μL of Lipofectamine per well, following the kit’s instructions.

    Article Title: SUMOylation inhibition enhances multiple myeloma sensitivity to lenalidomide.
    Article Snippet: Mycoplasma was routinely tested using Mycoplasma PCR detection kit (G238, Abcam). .. Plasmid MSCB-hDot1Lwt was a gift from Dr. Yi Zhang (Addgene plasmid # 74173; http://n2t.net/addgene:74173; RRID: Addgene_74173) [29]. .. MM1S cells were transfected by electroporation using Nucleofector 4D system and SE Cell Line 4D-NucleofectorTM X Kit L(Lonza).

    Expressing:

    Article Title: S-acylation regulates the membrane association and activity of Calpain-5.
    Article Snippet: The C-terminally 3×FLAG-tagged human CAPN6 expression construct was made by PCR amplification of the CAPN6 coding region from MGC clone 3503916 as template (Dharmacon, MHS6278202828128), and inserting it between the EcoRI and XbaI sites of p3×FLAG-CMV-14. .. The p3×FLAG-CAPN1 and p3×FLAG-CAPN2 expression constructs were gifts from Dr. Yi Zhang (Addgene plasmids # 60941 and 60942, http://n2t.net/addgene:60941, RRID:Addgene_60941, and http://n2t.net/addgene:60942, RRID:Addgene_60942 [40]). ..

    Construct:

    Article Title: S-acylation regulates the membrane association and activity of Calpain-5.
    Article Snippet: The C-terminally 3×FLAG-tagged human CAPN6 expression construct was made by PCR amplification of the CAPN6 coding region from MGC clone 3503916 as template (Dharmacon, MHS6278202828128), and inserting it between the EcoRI and XbaI sites of p3×FLAG-CMV-14. .. The p3×FLAG-CAPN1 and p3×FLAG-CAPN2 expression constructs were gifts from Dr. Yi Zhang (Addgene plasmids # 60941 and 60942, http://n2t.net/addgene:60941, RRID:Addgene_60941, and http://n2t.net/addgene:60942, RRID:Addgene_60942 [40]). ..

    Over Expression:

    Article Title: Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia
    Article Snippet: .. For Tet3 overexpression, we used a previously reported Tet3 plasmid by Dr. Yi Zhang, pcDNA-Flag-Tet3 (Addgene plasmid #60940; RRID:Addgene_60940 ) ( ); a pBluescript II KS(+) plasmid was used as a control plasmid for the transfection procedure. .. Lipofectamine 3,000 reagent (Thermo Fisher Scientific) was used for transfection, with 5 μL of Lipofectamine per well, following the kit’s instructions.

    Control:

    Article Title: Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia
    Article Snippet: .. For Tet3 overexpression, we used a previously reported Tet3 plasmid by Dr. Yi Zhang, pcDNA-Flag-Tet3 (Addgene plasmid #60940; RRID:Addgene_60940 ) ( ); a pBluescript II KS(+) plasmid was used as a control plasmid for the transfection procedure. .. Lipofectamine 3,000 reagent (Thermo Fisher Scientific) was used for transfection, with 5 μL of Lipofectamine per well, following the kit’s instructions.

    Transfection:

    Article Title: Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia
    Article Snippet: .. For Tet3 overexpression, we used a previously reported Tet3 plasmid by Dr. Yi Zhang, pcDNA-Flag-Tet3 (Addgene plasmid #60940; RRID:Addgene_60940 ) ( ); a pBluescript II KS(+) plasmid was used as a control plasmid for the transfection procedure. .. Lipofectamine 3,000 reagent (Thermo Fisher Scientific) was used for transfection, with 5 μL of Lipofectamine per well, following the kit’s instructions.



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    91
    Addgene inc dr yi zhang
    Dr Yi Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dr+yi+zhang/pcDNA-Flag-Tet3+(Plasmid+%2360940)/pmc12310658-89-11-15
    Average 91 stars, based on 1 article reviews
    dr yi zhang - by Bioz Stars, 2026-10
    91/100 stars
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